minocycline hydrochloride

Fingolimod potentiates minocycline against multidrug-resistant Elizabethkingia.

Sun Jie J, Feng Qiang Q, Yang Can C, Zong Yulong Y et al.

Elizabethkingia spp. are intrinsically resistant to multiple antimicrobial classes and are emerging nosocomial pathogens. This study evaluated the antibacterial activity of fingolimod against Elizabethkingia and its interaction with minocycline. Sixteen residual clinical isolates were examined. Baseline single-agent susceptibility was assessed by a single disk-diffusion determination and by VITEK 2. Fingolimod MIC and MBC values were measured by broth microdilution. Biofilm formation was quantified by crystal violet staining. Fingolimod-minocycline interactions were evaluated by checkerboard and time-kill assays. Preliminary protective activity was examined in a Galleria mellonella model infected with EA12. Disk diffusion and VITEK 2 showed method-dependent discordance for piperacillin-tazobactam. Fifteen of 16 isolates formed biofilm; 13 of these 15 were weak and two were moderate producers. Fingolimod MICs ranged from 6.25 to 25 μg/mL. Fingolimod reduced the minocycline MIC by 4-8-fold in six synergistic isolates. The combination produced preliminary, isolate-specific protection in Galleria mellonella infected with EA12. Fingolimod exhibits antibacterial activity against Elizabethkingia spp. and potentiates minocycline in a subset of isolates. These findings support further development of fingolimod-based combination strategies and optimization of the fingolimod scaffold.

Effectiveness and Safety of a Moisturizer Containing Centella Asiatica Extract, Ceramide NP, and Panthenol for Erythema and Skin Barrier in Erythematotelangiectatic Rosacea: A Randomized Controlled Trial Study.

Jiang Xia X, Luo Haoyun H, He Xiao X, Hu Chenmin C et al.

Erythematotelangiectatic rosacea is a chronic recurrent inflammatory disease that often impose a significant psychological burden on patients. Given that effective moisturization is integral to its management, the choice of a proper moisturizer is a critical clinical consideration. Our study aims to assess the effectiveness and safety of a moisturizer containing Centella asiatica extract, ceramide NP, and panthenol for erythema and skin barrier in Chinese patients with erythematotelangiectatic rosacea. All enrolled patients were prescribed oral minocycline 50 mg once daily and applied moisturizer twice daily. Facial images of facial redness, stratum corneum hydration (SCH), transepidermal water loss (TEWL), and Clinician's Erythema Assessment (CEA) were collected at baseline, day 7 (D7), day 14 (D14), and day 28 (D28). Time to a 1-point reduction in the Patient's Self-Assessment (PSA) scale and Rosacea-specific Quality-of-life instrument (RosQol) were also recorded by patients. A total of 75 patients with rosacea were enrolled, 39 patients were randomized to the experimental group and 36 to the control group. Our study showed that the variations of a* value, facial red area, SCH and RosQol from baseline to D28 were rapidly and significantly greater in the experimental group, and the mean rank of CEA was significantly higher in the control group at D28. All AEs were mild and self-limiting. This study found that the moisturizer containing Centella asiatica extract, ceramide NP, and panthenol delivers rapid, effective adjunctive benefits in rosacea treatment and represents a reliable daily skincare option for patients.

Correction to: In vitro comparative quality evaluation of different brands of metformin hydrochloride tablets available in Mekelle City, Tigray Regional State, Ethiopia.

Welegebrial Brhane Gebrehiwot BG, Gezae Zenawi Z, Gebrekidan Samrawit Kidanemariam SK, Gebrerufael Gebru Gebremeskel GG et al.

Activating systemic inflammation with lipopolysaccharide drives microglia-mediated degradation of perineuronal nets after cervical spinal cord injury in female rats.

Cucarian Jaison J, Rakheja Ram R, Scatterty Kale R KR, Armstrong Edward E et al.

Functional gains from rehabilitative motor training after spinal cord injury (SCI) depend on neuroplasticity, which naturally declines over time. We previously demonstrated that combining the pro-inflammatory compound lipopolysaccharide (LPS) with motor training reawakens neuroplasticity and enhances its therapeutic efficacy in chronic cervical SCI rats, though the underlying mechanisms remained unclear. Here, we investigated whether LPS-induced inflammation facilitates neuroplasticity by degrading perineuronal nets (PNNs), extracellular structures known to restrict recovery after SCI. Adult female Lewis rats received a single intraperitoneal LPS injection (1.5 mg/kg) ten days post-cervical SCI. In the first experiment, five days post-injection, we observed significantly diminished PNN marker fluorescence around ventral horn motor neurons in spared, injury-reactive spinal cord regions. To determine the cellular mechanism, we conducted a second experiment in which we pharmacologically attenuated microglial activity using minocycline (35 mg/kg). Minocycline treatment successfully prevented the LPS-induced loss of PNN fluorescence, demonstrating that microglia actively mediate PNN breakdown. Collectively, these findings provide a proof-of-concept mechanism by which inflammation influences neuroplasticity following central nervous system injury. Our observations support the growing recognition that inflammation, far from being exclusively detrimental, holds the capacity to contribute to healing after injury. By activating microglia to degrade PNNs, LPS-induced inflammation removes the structural barrier surrounding motor neurons, potentially priming them to be more responsive to the neuroplastic changes induced by rehabilitative motor training, providing a plausible explanation for our previously reported outcomes. This study highlights neuroimmune interactions as key regulators of neuroplasticity and the therapeutic efficacy of rehabilitative interventions in neurological disorders.