Factor VIII (ReFacto AF / Xyntha)

Psychometric assessment and measurement invariance of the Intuitive Eating Scale-3 among Brazilian men and women.

Berbert de Carvalho Pedro Henrique PH, Tylka Tracy L TL, Resende Thainá Richelli Oliveira TRO, de Fialho Daena Cunha DC et al.

Intuitive eating is an adaptive approach to eating that uniquely contributes to improved quality of life and psychological well-being. The Intuitive Eating Scale is a widely used measure that has been validated across diverse cultural contexts and among individuals of different sociocultural backgrounds. However, its third version (IES-3) was only tested among U.S. adults. In the present study, we perform a psychometric assessment of the Brazilian Portuguese translation of the IES-3 in a sample of 522 men and women. We evaluated the factor structure using a two-step, split-sample exploratory (n = 254) and confirmatory factor (CFA) analytic approach. CFA and exploratory structural equation modeling supported the original four-factor structure of the measure. Bifactor CFA confirms the existence of an overarching factor. Full measurement invariance of the IES-3 was found between men and women. Additionally, we found good convergent validity with measures of disordered eating behaviors, positive eating, body appreciation, and self-compassion. Finally, good internal consistency was obtained for the IES-3 total scores and its four subscales. Collectively, these results support the use of the measure in Brazilian men and women and provide a foundation to expand the literature on eating disorder prevention, body image, and mental health in this population.

Correction to "Stromal Cell-Derived Factor-1α Alleviates Calcium-Sensing Receptor Activation-Mediated Ischemia/Reperfusion Injury by Inhibiting Caspase-3/Caspase-9-Induced Cell Apoptosis in Rat Free Flaps".

Psychometric Evaluation of the Parental Education in Physiotherapy Scale: A Brief Measure of Family-Centered Educational Practices in Pediatric Physiotherapy.

Pacheco-Molero Manuel M, Gutiérrez-Ortega Mónica M, Morales-Murillo Catalina C, León-Estrada Irene I

To evaluate the psychometric properties of the Parental Education in Physiotherapy (PEP) scale, designed to assess the extent to which pediatric physiotherapists implement family-centered educational practices and caregiver education strategies for families of children under 6 years of age. This is an instrumental cross-sectional study based on a digital survey targeting physiotherapists in Spain. A sequential cross-validation procedure was applied by randomly dividing the total sample (N = 310) to two equivalent subsamples (n = 155/155). The final 8-item set was selected in Subsample 1 based exclusively on the EFA results and theoretical considerations, before the CFA results were examined. The prespecified 8-item, 3-factor structure was subsequently evaluated through CFA in Subsample 2. The CFA was estimated using diagonally weighted least squares (DWLS). Convergent validity evidence was examined using average variance extracted (AVE), and discriminant validity evidence was examined using inter-factor correlations, while associations with the use of technological devices for motor tracking were analyzed as an exploratory external comparison. Internal consistency was estimated using reliability coefficients appropriate for ordinal data. A total of 310 physiotherapists participated (mean age = 36.4 years; 87.4% women). The final 8-item, 3-factor model (Collaboration, Capacity-Building, and Reflection) showed excellent fit (CFI = 1.000, RMSEA = 0.011, SRMR = 0.031), AVE values above 0.50 in all three factors (0.587-0.771), and latent factor correlations below 0.85 (0.40-0.64). Internal consistency was adequate for the three subscale scores. Reliability coefficients for the total composite score were also reported descriptively, although a global factor was not evaluated. The PEP is a brief measure with promising initial psychometric properties for describing and reflecting on family-centered educational practices and caregiver education in pediatric physiotherapy. Its three-dimensional structure and digital format may facilitate professional reflection, training, and quality- improvement initiatives within family-centered care models.

Loss of dmrt2a Alters Hepatic LC-PUFA Composition and Lipid Homeostasis in Zebrafish.

Hou Mingxi M, Li Donghao D, Zhang Yaqun Y, Li Hengde H

Long-chain polyunsaturated fatty acids (LC-PUFAs), particularly eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), are essential nutrients for human health, and fish and aquatic products are the primary dietary sources of these bioactive lipids. The endogenous capacity of teleosts to synthesize LC-PUFAs directly determines their nutritional value for human consumers. However, the genetic regulators governing this biosynthetic pathway remain largely unexplored. Here, we show that targeted disruption of dmrt2a, a transcription factor gene, in zebrafish (Danio rerio) via CRISPR/Cas9, alters hepatic LC-PUFA composition and lipid homeostasis. Homozygous dmrt2a-/- mutants exhibited significantly reduced molar percentages of EPA (C20:5n-3) and DHA (C22:6n-3) in the liver, accompanied by the accumulation of their precursors, C18:2n-6 and C18:3n-3. In addition, the mutants developed a hepatic lipid metabolic disorder characterized by pathological accumulation of lipid droplets and elevated triglyceride levels, thereby providing a novel zebrafish model for research on hepatic steatosis. Transcriptomic profiling revealed that differentially expressed genes between dmrt2a-/- mutant and wild-type livers were significantly enriched in fatty acid biosynthesis and metabolic pathways. Notably, an LC-PUFA-enriched diet partially rescued the aberrant hepatic lipid phenotype. To our knowledge, this is the first study in any fish species to link a DMRT-family transcription factor to hepatic LC-PUFA composition and lipid homeostasis, suggesting dmrt2a as a potential target for genetic breeding to improve the nutritional quality of aquaculture products.